Effects of sodium bicarbonate, potassium chloride and sodium chloride supplementation on some blood biochemical parameters in laying hens

*Corresponding author: [email protected] ___________________________________________________________________________ This research was carried out to determine the effect of supplementation of sodium bicarbonate (% 0.38, % 1.5), potassium chloride (% 0.7) and sodium chloride (% 0.25) at different levels in diets of 480, 25-week old laying hens on blood plasma Ca, Pi, Mg, CI, Na, K, total protein, albumin and uric acid levels. During the 90-day trial, animals were divided into eight groups, 60 animals in each. Then each group was divided into six subgroups, 10 animals in each. Animals had access to ad libitum feed and water. At the end of the experimental period, blood samples of six chicks from each group were collected into heparinized tubes and plasma were separated by centrifugation at 2500 rpm for 10 min at +4 Co. Na and K concentration were quantified by flame fotometry and Ca, Pi, Mg, CI, total protein, albumin ve uric acid concentration were measured by spectrophotometry. Data was analysed using variance analysis and Duncan Multiple Range Test. Supplementation of diet with sodium bicarbonate, potassium chloride and sodium chloride did not affect plasma Ca and uric acid concentrations between control and treatment groups. However, Pi, Mg, CI, Na, K, total protein, and albumin concentrations differed statistically among groups due to the diet supplementations. As a result, in laying hen diet, supplementation of sodium bicarbonate, potassium chloride and sodium chloride either alone or combined caused important changes in some biochemical values. Detection of interaction among minerals warrants further investigations. __________________________________________________________________________________